The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases

Mol Cell. 2000 Dec;6(6):1377-87. doi: 10.1016/s1097-2765(00)00135-0.

Abstract

We describe a novel set of oscillation mechanisms for the fission yeast S phase cyclin Cig2, which contains an authentic destruction box and is destroyed at anaphase via the APC/cyclosome (APC/C). Unlike the mitotic cyclin Cdc13, however, Cig2 mRNA and protein peak at the G1/S boundary and decline to low levels in G2 and M phases. We show here that SCF(Pop1, Pop2) plays a role in transcriptional periodicity, as pop mutations result in constitutive cig2(+) transcripts. The instability of Cig2 during G2 and M is independent of either the APC/C or Pop1/Pop2, but requires Skp1, a core component of SCF. These data indicate that the APC/C and SCF control Cig2 levels differentially at different stages of the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase
  • Anaphase-Promoting Complex-Cyclosome
  • Animals
  • CDC2 Protein Kinase / metabolism
  • Cell Cycle Proteins*
  • Cyclin B / metabolism
  • Cyclins / genetics
  • Cyclins / metabolism*
  • Cysteine Endopeptidases / metabolism
  • Fluorescent Antibody Technique
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • G1 Phase*
  • G2 Phase
  • Gene Expression Regulation, Fungal*
  • Half-Life
  • Ligases / metabolism*
  • Mitosis
  • Multienzyme Complexes / metabolism
  • Mutation
  • Peptide Synthases / metabolism*
  • Proteasome Endopeptidase Complex
  • Protein Binding
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Ribonucleases*
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / metabolism
  • S Phase*
  • SKP Cullin F-Box Protein Ligases
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces pombe Proteins*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Ubiquitin-Protein Ligase Complexes*
  • Ubiquitin-Protein Ligases
  • Xenopus

Substances

  • Cell Cycle Proteins
  • Cig2 protein, S pombe
  • Cyclin B
  • Cyclins
  • Fungal Proteins
  • Multienzyme Complexes
  • RNA, Messenger
  • Ribonucleoproteins
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • pop1 protein, S pombe
  • Ubiquitin-Protein Ligase Complexes
  • Anaphase-Promoting Complex-Cyclosome
  • SKP Cullin F-Box Protein Ligases
  • Ubiquitin-Protein Ligases
  • CDC2 Protein Kinase
  • Ribonucleases
  • POP2 protein, S cerevisiae
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex
  • Ligases
  • Peptide Synthases