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Biochem Biophys Res Commun. 2001 Feb 9;280(5):1316-22.

Upregulation of HSP47 and collagen type III in the dermal fibrotic disease, keloid.

Author information

1
Department of Molecular and Cellular Biology, Kyoto University, Kyoto, 606-8397, Japan.

Abstract

Keloid is a dermal fibrotic disease characterized by excessive accumulation of mainly type I collagen in extracellular matrix of the dermis. We have studied the expression levels of collagen types I and III, and its molecular chaperone HSP47 in keloid lesions and surrounding unaffected skin using Northern and Western blotting and immunohistochemical analyses. Collagen types I and III mRNA levels were found to be upregulated 20-fold in keloid tissues, contradicting previous reports of nearly normal type III collagen levels in this disease. HSP47 expression in keloid lesions was also highly upregulated; eightfold at mRNA level and more than 16-fold at the protein level. Strong upregulation of these three proteins in keloid was confirmed by immunohistochemical staining. These results suggest that accumulation of both type I and type III collagen is important for the development of keloid lesions, and that HSP47 plays a role in the rapid and extensive synthesis of collagen in keloid tissues.

PMID:
11162672
DOI:
10.1006/bbrc.2001.4257
[Indexed for MEDLINE]

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