Format

Send to

Choose Destination
Mol Ther. 2001 Jan;3(1):97-104.

Generation of a stable cell line producing high-titer self-inactivating lentiviral vectors.

Author information

1
Gene Therapy Center, University of North Carolina Chapel Hill, 7119 Thurston-Bowles, Chapel Hill, North Carolina 27599-7352, USA.

Abstract

To facilitate the generation of SIN lentivirus vector-producer cell lines, we have developed a novel conditional SIN (cSIN) lentivirus vector, which retains its SIN properties in normal target cells yet can be produced at high titers from tetracycline-regulated packaging cell lines. The design of the cSIN vector is based on replacing the vector U3 transcription regulatory elements with the Tet-responsive element, which allows vector production exclusively in cells expressing the synthetic Tet-regulated transactivator (tTA). In contrast minimal vector production ( approximately 200 IU/ml) is obtained in target cells that do not express the tTA, even in the presence of all HIV-1 proteins. Following transduction of the Tet-regulated SODk1 lentivirus vector-packaging cell line with the cSIN vector, high titers of cSIN recombinant vector (>10(6) IU/ml) could be generated, which efficiently transduced terminally differentiated neurons in normal rat brain.

PMID:
11162316
DOI:
10.1006/mthe.2000.0238
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center