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Science. 2001 Jan 19;291(5503):493-5.

Effects of cis arrangement of chromatin insulators on enhancer-blocking activity.

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1
Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.

Abstract

Chromatin boundary elements or insulators are believed to regulate gene activity in complex genetic loci by organizing specialized chromatin structures. Here, we report that the enhancer-blocking activity of the Drosophila suHw insulator is sensitive to insulator copy number and position. Two tandem copies of suHw were ineffective in blocking various enhancers from a downstream promoter. Moreover, an enhancer was blocked more effectively from a promoter by two flanking suHw insulators than by a single intervening one. Thus, insulators may modulate enhancer-promoter interactions by interacting with each other and facilitating the formation of chromatin loop domains.

PMID:
11161205
DOI:
10.1126/science.291.5503.493
[Indexed for MEDLINE]
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