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Antimicrob Agents Chemother. 2001 Feb;45(2):616-20.

Nucleotide sequence and characterization of a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-10) isolated in Spain.

Author information

1
Servicio de Microbiología, Hospital Ramón y Cajal, Madrid, Spain.

Abstract

A cefotaxime-resistant, ceftazidime-susceptible Escherichia coli isolate was obtained from a patient with sepsis in 1997, from which a beta-lactamase with a pI of 8.1 was cloned. Cephaloridine and cefotaxime relative hydrolysis rates were 167 and 81, respectively (penicillin G rate = 100), whereas ceftazidime hydrolysis was not detected. The nucleotide sequence revealed a bla gene related to that coding for CTX-M-3. Despite 21 nucleotide substitutions, only 2 determined amino acid changes (Ala27Val and Arg38Gln). The amino acid sequence identity between this enzyme, designated CTX-M-10, and the chromosomal beta-lactamase of Kluyvera ascorbata was 81%.

PMID:
11158766
PMCID:
PMC90338
DOI:
10.1128/AAC.45.2.616-620.2001
[Indexed for MEDLINE]
Free PMC Article

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