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J Clin Microbiol. 2001 Feb;39(2):765-8.

Quantitative detection of hepatitis C virus RNA by light cycler PCR and comparison with two different PCR assays.

Author information

1
Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, 20246 Hamburg, Germany. mschroet@uke.uni-hamburg.de

Abstract

The new Light Cycler technology was adapted to the detection of hepatitis C virus (HCV) RNA in clinical samples. Sera from 81 patients were tested by Light Cycler PCR, AMPLICOR HCV Monitor assay, and in-house PCR. Our data demonstrate that Light Cycler is a fast and reliable method for the detection and quantitation of HCV RNA.

PMID:
11158147
PMCID:
PMC87816
DOI:
10.1128/JCM.39.2.765-768.2001
[Indexed for MEDLINE]
Free PMC Article

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