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Biochim Biophys Acta. 1975 Jan 30;379(1):189-92.

A new principle in biospecific affinity chromatography used for purification of cobalamin-binding proteins.


To avoid using protein-denaturing agents for desorption, when purifying cobalamin-binding protein by biospecific affinity chromatography, an affinity column has been prepared where cobalamin is attached through a temperature-labile linkage to insolubilized 3, 3'-diaminodipropylamine. On desorption the protein is obtained in solution saturated with cobalamin. The method has been used for purification of intrinsic factor and transcobalamin I.

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