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Appl Environ Microbiol. 2001 Feb;67(2):575-85.

gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication.

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1
Department of Microbiology, The Technical University of Denmark, DK-2800 Lyngby, Denmark.

Abstract

In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.

PMID:
11157219
PMCID:
PMC92623
DOI:
10.1128/AEM.67.2.575-585.2001
[Indexed for MEDLINE]
Free PMC Article
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