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Acta Virol. 2000 Jun-Aug;44(3):199-202.

Analysis of interaction between molecules of Bombyx mori nucleopolyhedrovirus IE-2 using a yeast two-hybrid system.

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1
Laboratory of Molecular Entomology and Baculovirology, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.

Abstract

Baculovirus IE-2 protein is one of well-known transactivators. In this report, we demonstrate that Bombyx mori nucleopolyhedrovirus (BmNPV) IE-2 interacts with itself. Several clones were obtained from a yeast two-hybrid screening system using IE-2 as bait and were found to encode IE-2 protein. Nucleotide sequencing of these clones showed that they contained C-terminal regions in common. Further analyses suggest that BmNPV IE-2 protein interacts with itself through 80 amino acid residues of coiled-coil domain in C-terminus.

PMID:
11155366
[Indexed for MEDLINE]
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