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Anal Chem. 2000 Dec 15;72(24):5881-5.

Internal calibration on adjacent samples (InCAS) with Fourier transform mass spectrometry.

Author information

1
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA. poconnor@bu.edu

Abstract

Using matrix-assisted laser desorption/ionization (MAL DI) on a trapped ion mass spectrometer such as a Fourier transform mass spectrometer (FTMS) allows accumulation of ions in the cell from multiple laser shots prior to detection. If ions from separate MALDI samples are accumulated simultaneously in the cell, ions from one sample can be used to calibrate ions from the other sample. Since the ions are detected simultaneously in the cell, this is, in effect, internal calibration, but there are no selective desorption effects in the MALDI source. This method of internal calibration with adjacent samples is demonstrated here on cesium iodide clusters, peptides, oligosaccharides, poly(propylene glycol), and fullerenes and provides typical FTMS internal calibration mass accuracy of < 1 ppm.

PMID:
11140752
DOI:
10.1021/ac000770t
[Indexed for MEDLINE]

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