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Bioorg Med Chem Lett. 2001 Jan 8;11(1):39-42.

Design and synthesis of rhodamine 110 derivative and caspase-3 substrate for enzyme and cell-based fluorescent assay.

Author information

1
Maxim Pharmaceuticals, San Diego, CA 92121, USA. scai@maxim.com

Abstract

N-Octyloxycarbonyl-R110 (1), with enhanced cell penetration and retention properties, was prepared from rhodamine 110. The tetrapeptide substrate N-Ac-DEVD-N'-octyloxycarbonyl-R110 (3) was prepared and shown to be efficiently cleaved by human recombinant caspase-3 and by apoptotic HL-60 cells. This substrate should prove useful in cell-based assays for apoptosis inducers and inhibitors.

PMID:
11140728
DOI:
10.1016/s0960-894x(00)00590-4
[Indexed for MEDLINE]

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