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Curr Microbiol. 2001 Feb;42(2):96-9.

PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins.

Author information

1
Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel. ariehz@bgumail.bgu.ac.il

Abstract

An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.

PMID:
11136129
[Indexed for MEDLINE]

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