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Curr Microbiol. 2001 Feb;42(2):96-9.

PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins.

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Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel.


An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.

[Indexed for MEDLINE]

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