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Curr Microbiol. 2001 Feb;42(2):78-81.

The release of alginate lyase from growing Pseudomonas syringae pathovar phaseolicola.

Author information

1
Audubon Sugar Institute, Louisiana Experimental Station, South Stadium Road, Baton Rouge, Louisiana 70803, USA. charlie.m.ott1@jsc.nasa.gov

Abstract

Pseudomonas syringae pathovar phaseolicola, which produces alginate during stationary growth phase, displayed elevated extracellular alginate lyase activity during both mid-exponential and late-stationary growth phases of batch growth. Intracellular activity remained below 22% of the total activity during exponential growth, suggesting that alginate lyase has an extracellular function for this organism. Extracellular enzyme activity in continuous cultures, grown in either nutrient broth or glucose-simple salts medium, peaked at 60% of the washout rate, although nutrient broth-grown cultures displayed more than twice the activity per gram of cell mass. These results imply that growth rate, nutritional composition, or both initiate a release of alginate lyase from viable P. syringae pv. phaseolicola, which could modify its entrapping biofilm.

PMID:
11136126
[Indexed for MEDLINE]

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