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J Biol Chem. 2001 Apr 20;276(16):12654-9. Epub 2000 Dec 21.

SUMO-1 conjugation in vivo requires both a consensus modification motif and nuclear targeting.

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1
School of Biology, Biomolecular Sciences Building, University of St. Andrews, St. Andrews, Fife KY169ST, United Kingdom.

Abstract

SUMO-1 is a small ubiquitin-related modifier that is covalently linked to many cellular protein targets. Proteins modified by SUMO-1 and the SUMO-1-activating and -conjugating enzymes are located predominantly in the nucleus. Here we define a transferable sequence containing the PsiKXE motif, where Psi represents a large hydrophobic amino acid, that confers the ability to be SUMO-1-modified on proteins to which it is linked. Whereas addition of short sequences from p53 and IkappaBalpha, containing the PsiKXE motif, to a carrier protein is sufficient for modification in vitro, modification in vivo requires the additional presence of a nuclear localization signal. Thus, protein substrates must be targeted to the nucleus to undergo SUMO-1 conjugation.

PMID:
11124955
DOI:
10.1074/jbc.M009476200
[Indexed for MEDLINE]
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