Cloning and functional expression of the rat alpha(2) subunit of soluble guanylyl cyclase

M Koglin; S Behrends

doi:10.1016/s0167-4781(00)00211-6

Cloning and functional expression of the rat alpha(2) subunit of soluble guanylyl cyclase

Biochim Biophys Acta. 2000 Dec 1;1494(3):286-9. doi: 10.1016/s0167-4781(00)00211-6.

Authors

M Koglin¹, S Behrends

Affiliation

¹ Institut für Experimentelle und Klinische Pharmakologie, Universität Hamburg, Martinistrasse 52, D-20251 Hamburg, Germany.

PMID: 11121588
DOI: 10.1016/s0167-4781(00)00211-6

Abstract

Nitric oxide-sensitive guanylyl cyclase is a heterodimeric enzyme consisting of one alpha and one beta subunit. Here, we clone the first alpha(2) subunit ortholog and functionally express the cDNA in Sf-9 cells. Our data indicate a high degree of conservation of the primary sequence and functional activity of the rat alpha(2) subunit.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Cell Line
Cloning, Molecular
Conserved Sequence
Guanylate Cyclase / biosynthesis
Guanylate Cyclase / chemistry
Guanylate Cyclase / genetics*
Isoenzymes / genetics
Kidney / metabolism
Male
Molecular Sequence Data
Nitric Oxide / pharmacology
Rats
Rats, Sprague-Dawley
Solubility

Substances

Isoenzymes
Nitric Oxide
Guanylate Cyclase

Associated data

GENBANK/AF109963