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Biochim Biophys Acta. 2000 Dec 15;1517(1):159-63.

Cloning and expression of cDNAs encoding two enzymes of the MEP pathway in Catharanthus roseus.

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Laboratoire de Biologie Moléculaire et Biochimie végétale, EA2106, Faculté de Pharmacie, Université de Tours, 31 avenue Monge, 37200 Tours, France.


Two periwinkle cDNAs (crdxr and crmecs) encoding enzymes of the non-mevalonate terpenoid pathway were characterized using reverse transcription-PCR strategy based on the design of degenerated oligonucleotides. The deduced amino acid sequence of crdxr is homologue to 1-deoxy-D-xylulose 5-phosphate reductoisomerases. Crmecs represents the first plant cDNA encoding a protein similar to the 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Escherichia coli. Expression of crdxr and crmecs genes was up-regulated in periwinkle cells producing monoterpenoid indole alkaloids. Involvement of the 2C-methyl-D-erythritol 4-phosphate pathway in alkaloid biosynthesis is discussed.

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