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EMBO J. 2000 Dec 15;19(24):6918-23.

DNA of Drosophila melanogaster contains 5-methylcytosine.

Author information

1
Institut für Biochemie, Fachbereich 8, Heinrich-Buff-Ring 58, 35392 Giessen and Lehrstuhl für Genetik, Universität Bayreuth, Universitätstrasse 30, 95440 Bayreuth, Germany.

Abstract

It is commonly accepted that the DNA of Drosophila melanogaster does not contain 5-methylcytosine, which is essential in the development of most eukaryotes. We have developed a new, highly specific and sensitive assay to detect the presence of 5-methylcytosine in genomic DNA. The DNA is degraded to nucleosides, 5-methylcytosine purified by HPLC and, for detection by 1D- and 2D-TLC, radiolabeled using deoxynucleoside kinase and [gamma-(32)P]ATP. Using this assay, we show here that 5-methylcytosine occurs in the DNA of D. melanogaster at a level of approximately 1 in 1000-2000 cytosine residues in adult flies. DNA methylation is detectable in all stages of D.melanogaster development.

PMID:
11118227
PMCID:
PMC305887
DOI:
10.1093/emboj/19.24.6918
[Indexed for MEDLINE]
Free PMC Article

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