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Trends Biochem Sci. 2000 Dec;25(12):624-31.

Macromolecular electron microscopy in the era of structural genomics.

Author information

1
Dept of Molecular Structural Biology, Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152 Martinsried, Germany. baumeist@biochem.mpg.de

Abstract

Macromolecular machines carry out many cellular functions. Cryo-electron microscopy (cryo-EM) is emerging as a powerful method for studying the structure, assembly and dynamics of such macromolecules, and their interactions with substrates. With resolutions still improving, 'single-particle' analyses are already depicting secondary structure. Moreover, cryo-EM can be combined in several ways with X-ray diffraction to enhance the resolution of cryo-EM and the applicability of crystallography. Electron tomography holds promise for visualizing machines at work inside cells.

PMID:
11116190
DOI:
10.1016/s0968-0004(00)01720-5
[Indexed for MEDLINE]

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