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Trends Microbiol. 2000 Dec;8(12):571-7.

Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks.

Author information

1
Dept of Biomolecular Sciences, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, M60 1QD, Manchester, UK. finbarr.hayes@bi.umist.ac.uk

Abstract

Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity.

PMID:
11115754
DOI:
10.1016/s0966-842x(00)01857-6
[Indexed for MEDLINE]

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