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Ann Rheum Dis. 2001 Jan;60(1):49-54.

Recognition of YKL-39, a human cartilage related protein, as a target antigen in patients with rheumatoid arthritis.

Author information

1
Rheumatology, Immunology and Genetics Programme, Institute of Medical Science, St Marianna University, School of Medicine 2-16-1, Sugao, Miyamae-ku, Kawasaki, Kanagawa, 216-8512, Japan.

Abstract

OBJECTIVE:

To investigate whether autoimmunity to YKL-39, a recently cloned cartilage protein, occurs in patients with rheumatoid arthritis (RA).

METHODS:

Autoantibody to YKL-39 was assayed by enzyme linked immunosorbent assay (ELISA) and western blotting in serum samples from patients with RA, systemic lupus erythematosus (SLE), and healthy donors, using recombinant YKL-39 protein. This reactivity was compared with that against a YKL-39 homologue, YKL-40 (human cartilage gp-39/chondrex), which has been reported to be an autoantigen in RA.

RESULTS:

Autoantibody to YKL-39 was detected in seven of 87 patients with RA (8%), but not in serum samples from patients with SLE or healthy donors. YKL-40 reactivity was found in only one of 87 RA serum samples (1%), with no cross reactivity to YKL-39.

CONCLUSION:

The existence of anti-YKL-39 antibody in a subset of patients with RA is reported here for the first time. Further, it was shown that the immune response to YKL-39 was independent of that to YKL-40. Clarification of the antibody and T cell responses to autoantigens derived from chondrocyte, cartilage, or other joint components may lead to a better understanding of the pathophysiology of joint destruction in patients with RA.

PMID:
11114282
PMCID:
PMC1753367
DOI:
10.1136/ard.60.1.49
[Indexed for MEDLINE]
Free PMC Article

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