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Can J Microbiol. 2000 Nov;46(11):1042-5.

A factorial design analysis of chitin production by Cunninghamella elegans.

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Departamento de Patologia, Universidade Federal de Alagoas-UFAL, MaceiĆ³, Brasil.


Chitin production by Cunninghamella elegans (IFM 46109) was studied with a two-level full factorial design, varying time of cultivation and the concentration of D-glucose, L-asparagine, and thiamine in the culture medium. The material extracted was characterized by infrared and NMR spectroscopy. The highest chitin yield, 28.8%, was comparable with the highest in the literature and was obtained with a medium containing 60 g.L-1 of glucose, 3 g.L-1 of asparagine, and 0.008 mg.L-1 of thiamine. Increasing the time of cultivation from 24 h to 72 h did not affect chitin production. The three factors showed significant positive effects on chitin production, without interactions between them.

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