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J Chromatogr B Biomed Sci Appl. 2000 Sep 29;747(1-2):229-40.

Chromatographic and capillary electrophoretic methods for the analysis of nicotinic acid and its metabolites.

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Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.


Methods for the assay of nicotinic acid (NiAc) and its metabolites in biological fluids using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) are reviewed. Most of the references cited in this review concern HPLC methods. A few CE methods that have been recently reported are also included. As these compounds are relatively polar and have a wide range of physico-chemical properties, the sample pre-treatment or clean-up process prior to analysis is included. Most HPLC methods using an isocratic elution system allow determination of a single or few metabolites, but gradient HPLC methods enable simultaneous determination of five to eight compounds. Simultaneous determination of NiAc including many metabolites in a single run can be achieved by CE. We also discuss the pharmacokinetics of NiAc and some of its metabolites.

[Indexed for MEDLINE]

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