Format

Send to

Choose Destination
Curr Biol. 2000 Nov 16;10(22):1459-62.

The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo.

Author information

1
Ontario Cancer Institute/Amgen Institute, Department of Medical Biophysics, University of Toronto, 620 University Avenue, ON M5G 2C1, Toronto, Canada.

Erratum in

  • Curr Biol 2001 Jun 5;11(11):907.

Abstract

Mammalian telomerase is essential for the maintenance of telomere length [1-5]. Its catalytic core comprises a reverse transcriptase component (TERT) and an RNA component. While the biochemical role of mammalian TERT is well established [6-11], it is unknown whether it is sufficient for telomere-length maintenance, chromosome stability or other cellular processes. Cells from mice in which the mTert gene had been disrupted showed progressive loss of telomere DNA, a phenotype similar to cells in which the gene encoding the telomerase RNA component (mTR) has been disrupted [1,12]. On prolonged growth, mTert-deficient embryonic stem (ES) cells exhibited genomic instability, aneuploidy and telomeric fusions. ES cells heterozygous for the mTert disruption also showed telomere attrition, a phenotype that differs from heterozygous mTR cells [12]. Thus, telomere maintenance in mammals is carried out by a single, limiting TERT.

PMID:
11102810
DOI:
10.1016/s0960-9822(00)00805-8
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center