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Nat Neurosci. 2000 Dec;3(12):1256-65.

Presynaptic calcium stores underlie large-amplitude miniature IPSCs and spontaneous calcium transients.

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Arbeitsgruppe Zelluläre Neurobiologie, Max-Planck-Institut für biophysikalische Chemie, 37077 Göttingen, Germany.

Erratum in

  • Nat Neurosci 2001 Mar;4(3):331.


The cellular mechanisms responsible for large miniature currents in some brain synapses remain undefined. In Purkinje cells, we found that large-amplitude miniature inhibitory postsynaptic currents (mIPSCs) were inhibited by ryanodine or by long-term removal of extracellular Ca2+. Two-photon Ca2+ imaging revealed random, ryanodine-sensitive intracellular Ca2+ transients, spatially constrained at putative presynaptic terminals. At high concentration, ryanodine decreased action-potential-evoked rises in intracellular Ca2+. Immuno-localization showed ryanodine receptors in these terminals. Our data suggest that large mIPSCs are multivesicular events regulated by Ca2+ release from ryanodine-sensitive presynaptic Ca2+ stores.

[Indexed for MEDLINE]

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