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Biochem Biophys Res Commun. 2000 Nov 30;278(3):653-8.

A novel activating mutation of the K-ras gene in human primary colon adenocarcinoma.

Author information

1
Department of Molecular Genetics, Second Department of Surgery, Okayama University Medical School, Shikata-cho 2-5-1, Okayama, 700-8558, Japan.

Abstract

We identified a novel type of point mutation at the 22nd codon of the K-ras gene in a primary colon cancer. The mutation was C to A transversion substituting lysine (AAG) for normal glutamine (CAG) codon. Biological activity of this mutant K-ras gene was tested by expression of full-length cDNA clones in NIH3T3 cells. Most of the K-ras Lys22-transfected cells exhibited an increased saturation density, a lower serum requirement, and transformed morphology reminiscent to the typical K-ras Val12 transformants. However, the tumorigenicity of K-ras Lys22 transformants in nude mice was significantly less potent than that of K-ras Val12 transformants; only a high copy number transformant produced tumors. Even though the activation is incomplete, the finding that the majority of tumor cells in the specimen carried the K-ras Lys22 mutation suggests that this mutation might be advantageous for growth of tumor cells in vivo.

PMID:
11095964
DOI:
10.1006/bbrc.2000.3839
[Indexed for MEDLINE]

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