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FEMS Microbiol Lett. 2000 Dec 1;193(1):149-54.

Analysis of cepA and other Bacteroides fragilis genes reveals a unique promoter structure.

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1
Department of Microbiology and Immunology, 600 Moye Blvd., East Carolina University School of Medicine, Greenville, NC 27858-4354, USA.

Abstract

There is little known about the sequences that mediate the initiation of transcription in Bacteroides fragilis, thus transcriptional start sites for 13 new genes were determined and a total of 23 promoter regions upstream of the start sites were aligned and similarities were noted. A region at about -7 contained a consensus sequence of TAnnTTTG and upstream in the region centered at about -33, another TTTG motif was found in the majority of promoters examined. Canonical, Escherichia coli, -10 and -35 consensus sequences were not readily apparent. Mutations within the -7 motif indicated the TTTG residues were essential since changes in this sequence reduced the promoter activity to that of a no promoter control in a chloramphenicol acetyl transferase transcriptional fusion model system. Additional fusion studies indicated that the -33 region was also necessary for full activity.

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