Format

Send to

Choose Destination
Mol Cell. 2000 Oct;6(4):769-80.

A histone variant, Htz1p, and a Sir1p-like protein, Esc2p, mediate silencing at HMR.

Author information

1
Unit on Chromatin and Transcription, NICHD/NIH, Bethesda, Maryland 20892, USA.

Abstract

Silencing at HMR requires silencers, and one of the roles of the silencer is to recruit Sir proteins. This work focuses on the function of Sir1p once it is recruited to the silencer. We have generated mutants of Sir1p that are recruited to the silencer but are unable to silence, and we have utilized these mutants to identify four proteins, Sir3p, Sir4p, Esc2p, and Htz1p, that when overexpressed, restored silencing. The isolation of Sir3p and Sir4p validated this screen. Molecular analysis suggested that Esc2p contributed to silencing in a manner similar to Sir1p and probably helped recruit or stabilize the other Sir proteins, while Htz1p present at HMR assembled a specialized chromatin structure necessary for silencing.

PMID:
11090616
DOI:
10.1016/s1097-2765(00)00076-9
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center