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Biochim Biophys Acta. 2000 Nov 30;1543(1):123-30.

Localization and topology of ratp28, a member of a novel family of putative steroid-binding proteins.

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Biochemie-Zentrum, Heidelberg (BZH), University of Heidelberg, Im Neuenheimer Feld, 328, D-69120, Heidelberg, Germany.


We have cloned ratp28, a membrane protein from rat liver homologous to the previously described hpr6.6, a putative steroid-binding protein in humans. Ratp28 has a type II topology as determined by protease digestion experiments on intact and detergent-solubilized membranes. Subcellular fractionation by sucrose density centrifugation revealed a distribution for ratp28 identical to Bip as a marker for membranes of the endoplasmic reticulum. In these experiments no association was found with markers for Golgi or plasma membranes, indicating that ratp28 is localized to the endoplasmic reticulum.

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