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Arch Virol. 2000;145(10):2211-6.

A convenient semi-quantitative method for the diagnosis of Epstein-Barr virus reactivation.

Author information

1
Unité Mixte de Recherche, 7565 UHP-CNRS, Faculté de Médecine, Vandoeuvre-lès-Nancy, France.

Abstract

A semi-quantitative determination of Epstein-Barr virus (EBV) viremia has been devised. Peripheral blood mononuclear cells are recovered by Ficoll gradient and numerated. Five microl aliquots of recovered cell suspension and 5 microl of two standard dilutions (containing 500 and 100 cells, respectively) are subjected to a nested polymerase chain reaction (PCR). This technique has been evaluated over 3 years for the follow-up of 45 patients attending the Bone Marrow Transplantation Unit of the "Centre Hospitalier et Universitaire de Nancy". EBV reactivation was diagnosed in 13 patients (28%). Positivity of PCR for 100 cells was found in 9 patients of whom 6 developed lymphoma or lymphoproliferative disorder. This technique is easy to perform and doesn't necessitate any specific material besides the one necessary for routine genic amplification.

PMID:
11087103
DOI:
10.1007/s007050070051
[Indexed for MEDLINE]

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