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Neuron. 2000 Oct;28(1):195-204.

Cysteine string protein regulates G protein modulation of N-type calcium channels.

Author information

1
Department of Physiology and Biophysics, University of Calgary, Alberta, Canada.

Abstract

Cysteine string proteins (CSPs) are secretory vesicle proteins bearing a "J domain" and a palmitoylated cysteine-rich "string" region that are critical for neurotransmitter release. The precise role of CSP in neurotransmission is controversial. Here, we demonstrate a novel interaction between CSP, receptor-coupled trimeric GTP binding proteins (G proteins), and N-type Ca2+ channels. G. subunits interact with the J domain of CSP in an ATP-dependent manner; in contrast, Gbetagamma subunits interact with the C terminus of CSP in both the presence and absence of ATP. The interaction of CSP with both G proteins and N-type Ca2+ channels results in a tonic G protein inhibition of the channels. In view of the crucial importance of N-type Ca2+ channels in presynaptic vesicle release, our data attribute a key role to CSP in the fine tuning of neurotransmission.

PMID:
11086994
DOI:
10.1016/s0896-6273(00)00096-9
[Indexed for MEDLINE]
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