Small molecule-based chemical affinity system for the purification of proteins

Biotechniques. 2000 Nov;29(5):1126-33. doi: 10.2144/00295pf01.

Abstract

A new chemical affinity system is described for the purification of proteins. The Linx Affinity Purification System enables researchers to quickly and easily bind a protein ligand to a chromatographic matrix and use the resulting affinity resin to purify a second protein from crude mixtures. The entire process takes approximately 2 h.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Infective Agents / pharmacology
  • Antibodies / immunology
  • Antibodies / isolation & purification
  • Boronic Acids / chemistry
  • Buffers
  • Cell Extracts / chemistry
  • Chromatography, Affinity / methods*
  • Electrophoresis, Polyacrylamide Gel
  • Enzymes, Immobilized / immunology
  • Epitopes / immunology
  • Hydrogen-Ion Concentration
  • Hydroxamic Acids / chemistry
  • Immune Sera / chemistry
  • Molecular Structure
  • Molecular Weight
  • Protein Binding / drug effects
  • Proteins / chemistry
  • Proteins / immunology
  • Proteins / isolation & purification*
  • Proteins / metabolism
  • Rabbits
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / immunology
  • Recombinant Fusion Proteins / isolation & purification
  • Ribonuclease, Pancreatic / immunology
  • Ribonuclease, Pancreatic / metabolism
  • Salicylamides / chemistry
  • Sepharose / metabolism
  • Sterilization

Substances

  • Anti-Infective Agents
  • Antibodies
  • Boronic Acids
  • Buffers
  • Cell Extracts
  • Enzymes, Immobilized
  • Epitopes
  • Hydroxamic Acids
  • Immune Sera
  • Proteins
  • Recombinant Fusion Proteins
  • Salicylamides
  • salicylhydroxamic acid
  • Sepharose
  • Ribonuclease, Pancreatic