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Ann N Y Acad Sci. 2000;919:33-47.

Quantitative proteome analysis: methods and applications.

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  • 1Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.


With the completion of a rapidly increasing number of complete genomic sequences, much attention is currently focused on how the information contained in sequence databases might be interpreted in terms of the structure, function, and control of biological systems. Quantitative proteome analysis, the global analysis of protein expression, has been proposed as a method to study steady-state gene expression and perturbation-induced changes. Here, we discuss the rationale for quantitative proteome analysis, highlight the limitations in the current standard technology, and introduce a new experimental approach to quantitative proteome analysis.

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