Format

Send to

Choose Destination
Cell. 2000 Oct 27;103(3):457-66.

A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP.

Author information

1
Max-Planck-Institut für Biophysikalische Chemie, Abteilung Zelluläre Biochemie, Göttingen, Germany.

Abstract

The box C/D snoRNAs function in directing 2'-O-methylation and/or as chaperones in the processing of ribosomal RNA. We show here that Snu13p (15.5 kD in human), a component of the U4/U6.U5 tri-snRNP, is also associated with the box C/D snoRNAs. Indeed, genetic depletion of Snu13p in yeast leads to a major defect in RNA metabolism. The box C/D motif can be folded into a stem-internal loop-stem structure, almost identical to the 15.5 kD binding site in the U4 snRNA. Consistent with this, the box C/D motif binds Snu13p/ 15.5 kD in vitro. The similarities in structure and function observed between the U4 snRNP (chaperone for U6) and the box C/D snoRNPs raises the interesting possibility that these particles may have evolved from a common ancestral RNP.

PMID:
11081632
DOI:
10.1016/s0092-8674(00)00137-9
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center