Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393(T)

B Martínez; J Sillanpää; E Smit; T K Korhonen; P H Pouwels

doi:10.1128/JB.182.23.6857-6861.2000

Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393(T)

J Bacteriol. 2000 Dec;182(23):6857-61. doi: 10.1128/JB.182.23.6857-6861.2000.

Authors

B Martínez¹, J Sillanpää, E Smit, T K Korhonen, P H Pouwels

Affiliation

¹ Department of Applied Microbiology and Gene Technology, TNO Voeding, 3700 AJ Zeist, The Netherlands.

Abstract

The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393(T). The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA was presented at the surface, rendering the transformants able to bind to immobilized collagens.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / biosynthesis*
Bacterial Proteins / genetics
Cell Membrane / metabolism
Integrins / biosynthesis*
Integrins / genetics
Lacticaseibacillus casei / genetics
Lacticaseibacillus casei / metabolism*
Lactobacillus / genetics*
Membrane Glycoproteins*
Membrane Proteins / biosynthesis*
Membrane Proteins / genetics
Receptors, Collagen

Substances

Bacterial Proteins
Integrins
Membrane Glycoproteins
Membrane Proteins
Receptors, Collagen
S-layer proteins