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J Vet Med Sci. 2000 Oct;62(10):1109-10.

Sex determination by simultaneous amplification of equine SRY and amelogenin genes.

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Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association, Utsunomiya.


A quick method for sex determination of horses was developed. Simultaneous amplification of the equine sex-determining region of the Y chromosome gene (SRY) and amelogenin gene (AMEL) accomplished the determination of the presence of both the Y chromosome and SRY gene. In agarose gel electrophoresis, a normal stallion showed 1 SRY band and 3 AMEL (AMELX, AMELY, and AMELX/AMELY heteroduplex) bands, and a normal mare showed a single AMELX band. In XY-mares, 3 AMEL bands were detected as in a normal stallion, but no SRY band. The present method enables a quick diagnosis for XY-mare prior to cytogenetic analysis.

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