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Nucleic Acids Res. 2000 Nov 15;28(22):4531-9.

DNA-triplex stabilizing properties of 8-aminoguanine.

Author information

1
Departament de Bioquímica i Biologia Molecular, Facultat de Química, Universitat de Barcelona, Martí i Franquès 1, Barcelona 08028, Spain.

Abstract

A DNA-triplex stabilizing purine (8-aminoguanine) is designed based on molecular modeling and synthesized. The substitution of guanine by 8-aminoguanine largely stabilizes the triplex both at neutral and acidic pH, as suggested by molecular dynamics and thermodynamic integration calculations, and demonstrated by melting experiments. NMR experiments confirm the triplex-stabilizing properties of 8-aminoguanine and demonstrate that few changes are found in the structure of the triplex due to the presence of this modified base.

PMID:
11071942
PMCID:
PMC113881
[Indexed for MEDLINE]
Free PMC Article
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