Two nuclear localization signals are required for nuclear translocation of nuclear factor 1-A

FEBS Lett. 2000 Nov 3;484(2):118-24. doi: 10.1016/s0014-5793(00)02119-0.

Abstract

Nuclear factor 1 (NF1) proteins are encoded by at least four genes (NF1-A, B, C, X). Although DNA-binding and the transcription regulation domains of these proteins are well characterized, the nuclear localization signals (NLSs) are still unknown in all NF1s. We have identified two NLSs in NF1-A, and both are required for full translocation to the nucleus, although one of them itself has a partial translocation ability. These two NLSs are conserved in all four NF1s. Interestingly, three isoforms of NF1-A (NF1-A1, A2, A4) have two NLSs and translocate completely to the nucleus. In contrast, NF1-A3 lacks the second NLS and partially stays in the cytoplasm. Since NF1s construct homodimer and heterodimer, these findings indicate the differential regulations of the NF1 translocation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / metabolism
  • CCAAT-Enhancer-Binding Proteins / genetics
  • CCAAT-Enhancer-Binding Proteins / metabolism*
  • Cell Nucleus / metabolism*
  • DNA-Binding Proteins*
  • Exons
  • Green Fluorescent Proteins
  • HeLa Cells
  • Humans
  • Luminescent Proteins / metabolism
  • Molecular Sequence Data
  • NFI Transcription Factors
  • Nuclear Localization Signals / physiology*
  • Nuclear Proteins
  • Peptides / metabolism
  • Protein Transport
  • Transcription Factors*
  • Transfection
  • Y-Box-Binding Protein 1

Substances

  • Amino Acids
  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • Luminescent Proteins
  • NFI Transcription Factors
  • Nuclear Localization Signals
  • Nuclear Proteins
  • Peptides
  • Transcription Factors
  • Y-Box-Binding Protein 1
  • YBX1 protein, human
  • Green Fluorescent Proteins