Abstract
Nuclear factor 1 (NF1) proteins are encoded by at least four genes (NF1-A, B, C, X). Although DNA-binding and the transcription regulation domains of these proteins are well characterized, the nuclear localization signals (NLSs) are still unknown in all NF1s. We have identified two NLSs in NF1-A, and both are required for full translocation to the nucleus, although one of them itself has a partial translocation ability. These two NLSs are conserved in all four NF1s. Interestingly, three isoforms of NF1-A (NF1-A1, A2, A4) have two NLSs and translocate completely to the nucleus. In contrast, NF1-A3 lacks the second NLS and partially stays in the cytoplasm. Since NF1s construct homodimer and heterodimer, these findings indicate the differential regulations of the NF1 translocation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acids / metabolism
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CCAAT-Enhancer-Binding Proteins / genetics
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CCAAT-Enhancer-Binding Proteins / metabolism*
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Cell Nucleus / metabolism*
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DNA-Binding Proteins*
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Exons
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Green Fluorescent Proteins
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HeLa Cells
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Humans
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Luminescent Proteins / metabolism
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Molecular Sequence Data
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NFI Transcription Factors
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Nuclear Localization Signals / physiology*
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Nuclear Proteins
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Peptides / metabolism
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Protein Transport
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Transcription Factors*
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Transfection
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Y-Box-Binding Protein 1
Substances
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Amino Acids
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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Luminescent Proteins
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NFI Transcription Factors
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Nuclear Localization Signals
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Nuclear Proteins
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Peptides
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Transcription Factors
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Y-Box-Binding Protein 1
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YBX1 protein, human
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Green Fluorescent Proteins