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Thromb Haemost. 2000 Oct;84(4):657-63.

Tissue factor encryption/de-encryption is not altered in the absence of the cytoplasmic domain.

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  • 1Department of Pathology and Microbiology, University of Nebraska Medical Center, Nebraska Medical Center, Omaha 68198-6495, USA. 73632.3623@compuserve.com

Abstract

Since the cytoplasmic domain of tissue factor (TF) appears to have a role in TF function beyond coagulation, experiments were conducted to determine whether the cytoplasmic domain also has a role in regulating procoagulant activity of TF present in the cell membrane. TF encryption was quantitated in human YU-SITI, U87-MG, and mouse 3T3 cells which were transfected for expression of human tissue factor or a construct lacking the cytoplasmic domain (TF(CD)). Comparison of intact cells (encrypted) with fully disrupted cells (de-encrypted) showed that TF and TF(CD) were equally encrypted with respect to function in fX activation. Moreover, cells expressing TF and TF(CD) were indistinguishable in their procoagulant responses to A23187-calcium and varied concentrations of nonionic detergents. TF in membrane vesicles spontaneously shed by U87-MG cells was largely, but incompletely, de-encrypted, and the degree of de-encryption was independent of the cytoplasmic domain. We conclude that the predominant mechanism(s) for encrypting TF procoagulant activity is independent of the cytoplasmic domain.

PMID:
11057866
[PubMed - indexed for MEDLINE]
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