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Crit Care. 2000;4(4):255-61. Epub 2000 Jul 7.

Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid 'real-time' polymerase chain reaction.

Author information

1
Queen Astrid Military Hospital, Neder-Over-Heembeek, Belgium. skinbank@hmra.smd.be

Abstract

STATEMENT OF FINDINGS:

We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.

PMID:
11056755
PMCID:
PMC29046
[Indexed for MEDLINE]
Free PMC Article

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