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Fertil Steril. 2000 Nov;74(5):969-75.

Effect of hormonal agents on monocyte chemotactic protein-1 expression by endometrial epithelial cells of women with endometriosis.

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1
Unité d'Endocrinologie de la Reproduction, Centre de Recherche, H opital Saint-François d'Assise, Québec, Québec, Canada.

Abstract

OBJECTIVE:

To assess whether hormonal agents used in the medical treatment of endometriosis, such as danazol and GnRH agonist, exert direct regulatory action on monocyte chemotactic protein-1 (MCP-1) expression by endometrial epithelial cells.

DESIGN:

Primary cultures of epithelial cells isolated from human endometrium were exposed to different concentrations of cytokines and steroid hormone analogs. Expression of MCP-1 was analyzed at the levels of protein and messenger RNA.

SETTING:

Gynecology clinic and laboratory of endocrinology of reproduction.

PATIENT(S):

Women presenting for infertility or pelvic pain in whom endometriosis was diagnosed by using laparoscopy.

INTERVENTION(S):

Endometrial tissue biopsy performed at laparoscopy.

MAIN OUTCOME MEASURE(S):

Secretion of MCP-1 protein was measured by using enzyme-linked immunosorbent assay, and mRNA steady-state levels were measured by performing Northern blot analysis.

RESULT(S):

Buserelin acetate, a GnRH agonist (0.1-10 ng/mL), had no significant effect on MCP-1 expression, whereas danazol (10(-7)-10(-5) M), a testosterone analog, and dexamethasone, an anti-inflammatory glucocorticoid hormone (10(-12)-10(-6)M), showed a direct and a dose-dependent inhibitory effect on MCP-1 expression. This effect occurred at the level of protein and mRNA.

CONCLUSION(S):

The findings of the study may affect understanding of the mechanisms by which hormonal treatments act on endometriosis and influence its clinical manifestations.

PMID:
11056242
[Indexed for MEDLINE]
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