Format

Send to

Choose Destination
Neuron. 2000 Sep;27(3):525-37.

The gain of rod phototransduction: reconciliation of biochemical and electrophysiological measurements.

Author information

1
Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA.

Abstract

We have resolved a central and long-standing paradox in understanding the amplification of rod phototransduction by making direct measurements of the gains of the underlying enzymatic amplifiers. We find that under optimized conditions a single photoisomerized rhodopsin activates transducin molecules and phosphodiesterase (PDE) catalytic subunits at rates of 120-150/s, much lower than indirect estimates from light-scattering experiments. Further, we measure the Michaelis constant, Km, of the rod PDE activated by transducin to be 10 microM, at least 10-fold lower than published estimates. Thus, the gain of cGMP hydrolysis (determined by kcat/Km) is at least 10-fold higher than reported in the literature. Accordingly, our results now provide a quantitative account of the overall gain of the rod cascade in terms of directly measured factors.

PMID:
11055435
DOI:
10.1016/s0896-6273(00)00063-5
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center