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Dermatology. 2000;201(2):111-7.

Investigation of annexin V binding to lymphocytes after extracorporeal photoimmunotherapy as an early marker of apoptosis.

Author information

1
Institute of Immunology, Otto von Guericke University, Magdeburg, Germany. anneget.gerber@medizin.uni-magdeburg.de

Abstract

BACKGROUND:

Induction of programmed cell death is assumed to be a possible effect of extracorporeal photoimmunotherapy (ECPI).

OBJECTIVE:

In the present study lymphocytes of patients with cutaneous T cell lymphoma undergoing ECPI were investigated for early apoptotic events.

METHODS:

Annexin V, known for its selective affinity to phospholipids, was used to detect early phases of apoptosis. Simultaneous staining with propidium iodide binding to DNA allowed detection of late apoptotic/necrotic cells.

RESULTS:

At 1 h after ECPI, an increase in early apoptotic cells was found indicating a direct effect of ECPI. At 20 h after each ECPI session, a delayed increase in the number of apoptotic lymphocytes was observed in early apoptotic annexin-stained cells and in late apoptotic cells, whereas in nonirradiated cells no remarkable changes were found. Apoptosis was confirmed by altered light scattering properties and DNA fragmentation.

CONCLUSION:

The apoptotic cell death of reinfused lymphocytes is supposed to be a therapeutic effect of ECPI.

PMID:
11053912
DOI:
10.1159/000018472
[Indexed for MEDLINE]

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