Format

Send to

Choose Destination
J Biol Chem. 2001 Jan 26;276(4):2733-41. Epub 2000 Oct 25.

JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells.

Author information

1
Department of Pathology, Centre Medical Universitaire, 1 rue Michel-Servet, CH-1211 Geneva, Switzerland. michel.aurrand-lions@medecine.unige.ch

Abstract

Cell-cell contacts are essential for morphogenesis and tissue function and play a vital role in mediating endothelial cohesion within the vascular system during vessel growth and organization. We identified a novel junctional adhesion molecule, named JAM-2, by a selective RNA display method, which allowed identification of transcripts encoding immunoglobulin superfamily molecules regulated during coculture of endothelial cells with tumor cells. The JAM-2 transcript is highly expressed during embryogenesis and is detected in lymph node and Peyer's patches RNA of adult mice. Accordingly, antibodies specific for JAM-2 stain high endothelial venules and lymphatic vessels in lymphoid organs, and vascular structures in the kidney. Using real time video microscopy, we show that JAM-2 is localized within minutes to the newly formed cell-cell contact. The role of the protein in the sealing of cell-cell contact is further suggested by the reduced paracellular permeability of cell monolayer transfected with JAM-2 cDNA, and by the localization of JAM-2 to tight junctional complexes of polarized cells. Taken together, our results suggest that JAM-2 is a novel vascular molecule, which participates in interendothelial junctional complexes.

PMID:
11053409
DOI:
10.1074/jbc.M005458200
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center