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Invest Ophthalmol Vis Sci. 2000 Nov;41(12):4014-21.

Colocalization of TrkB and brain-derived neurotrophic factor proteins in green-red-sensitive cone outer segments.

Author information

1
Center for Research in Neuroscience, Montréal General Hospital Research Institute and McGill University, Montréal, Quebec, Canada. dipoloa@patho.umontreal.ca

Abstract

PURPOSE:

To examine the distribution of neurotrophins (NTs) and their catalytic receptors in adult rat photoreceptors.

METHODS:

Immunocytochemistry and Western blot analyses were performed using primary antibodies raised against NTs (nerve growth factor [NGF], brain-derived neurotrophic factor [BDNF], NT-3, and NT-4/5) and NT receptors (TrkA, TrkB, TrkC, and p75NTR). Double-labeling of retinal sections with opsin-specific antibodies was performed to identify each photoreceptor type. Competitive experiments using excess recombinant NT or Trk receptors confirmed the binding specificity of each antibody.

RESULTS:

TrkB and BDNF immunoreactivity was colocalized in cone outer segments. TrkB and BDNF were detected in all green-red-sensitive cones, but not in blue-UV cones or rods, and other NTs and NT receptors were not detected in any of the photoreceptor types.

CONCLUSIONS:

The findings suggest a specific role for BDNF through its signaling receptor TrkB in the function and maintenance of green-red cones, the predominant cone type in the rat retina.

PMID:
11053307
[Indexed for MEDLINE]

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