Imaging Ellipsometry is an optical surface-sensitive method for the investigation of various aspects of biomolecules adsorbing mainly at reflecting metal surfaces and silicon surface. It has advantages of high sensitivity to layer-thickness, big area of view, high sampling speed, and high lateral resolution. It can be used for studying adsorption kinetics of biomolecules and detecting complex layer of antigen-antibody when antibody (or antigen) combined with antigen (or antibody) coated on solid surfaces. Compared with other solid phase methods such as enzyme linked immunosorbent assay, immunofluorescence and radioimmunoassay, imaging ellipsometry has the advantage of not involving any labelling of reactants and it is a relatively inexpensive method and easy to handle. Compared with traditional ellipsometer, imaging ellipsometry shows an advantage of distinguishing both affinity and non-specific binding in different area on the surfaces.