Format

Send to

Choose Destination
Anal Biochem. 2000 Nov 1;286(1):80-5.

Effective isolation of high-quality total RNA from human adult articular cartilage.

Author information

1
Cartilage Research Group, Institute of Pathology, University of Erlangen-Nürnberg, Krankenhausstrasse 8-10, Erlangen, D-91054, Germany.

Abstract

The isolation of large quantities of good-quality RNA from human articular cartilage has been a long-standing problem for researchers working with human articular cartilage. In this paper we report a protocol which we have developed based on the Qiagen RNeasy procedure to produce high yields of purified, DNA-free RNA from normal and osteosteoarthritic human articular cartilage. The average yield of RNA was 8.39 microg/g (n = 59) for normal and 6.69 microg/g (n = 58) for osteoarthritic cartilage (average ratio OD 260/280 = 1.8-1.9). Quantitative PCR, cDNA array technology, and Northern blot analysis were used to verify the quality of the RNA.

PMID:
11038277
DOI:
10.1006/abio.2000.4779
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center