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Int Microbiol. 2000 Sep;3(3):159-64.

Pulsed-field gel electrophoresis analysis of virus assemblages present in a hypersaline environment.

Author information

1
Department of Marine Biology and Oceanography, Institute of Marine Sciences, CSIC, Barcelona, Spain. bdiez@icm.csic.es

Abstract

A method for analyzing virus assemblages in aquatic environments was developed and used for studying the highest-salinity ponds (from 13.4 to 35% salinity) from a multi-pond solar saltern in Alicante, Spain. The protocol consisted of a series of concentration and purification steps including tangential flow filtration and ultracentrifugation, followed by the preparation of total viral nucleic acids that were subsequently separated by pulsed-field gel electrophoresis. For every sample analyzed, a characteristic DNA pattern was obtained, whose complexity was related to viral diversity. The comparison of our results with a similar analysis carried out with marine virus assemblages shows that, as expected, the viral diversity corresponding to the analyzed hypersaline environment is considerably lower than that of a marine environment.

PMID:
11032308
[Indexed for MEDLINE]

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