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Proc Natl Acad Sci U S A. 1975 Aug;72(8):2910-4.

Presence of polyriboadenylate sequences in pulse-labeled RNA of Escherichia coli.

Abstract

Pulse-labeled RNA isolated from E. coli cells grown on limiting phosphate medium and phosphate-containing medium was analyzed by oligo(dT)-cellulose chromatography and by Millipore binding assay for polyriboadenylate-containing RNA. Whereas poly(A)-containing RNA amounted to as much as 15% of the total pulse-labeled RNA from cells grown on limiting phosphate medium, pulse-labeled RNA from cells grown on phosphate medium gave values around 1.5%. Steady-state labeled RNA from cells grown on limiting phosphate medium contained 1.2% poly(A) RNA. The addition of poly(A) sequences appears to be post-transcriptional. These results strongly favor the view that bacterial mRNAs may contain poly(A) stretches.

PMID:
1103127
PMCID:
PMC432888
[Indexed for MEDLINE]
Free PMC Article
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