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Annu Rev Cell Dev Biol. 2000;16:557-89.

Secretory protein trafficking and organelle dynamics in living cells.

Author information

1
Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA. jlippin@helix.nih.gov

Abstract

Green fluorescent protein chimerae acting as reporters for protein localization and trafficking within the secretory membrane system of living cells have been used in a wide variety of applications, including time-lapse imaging, double-labeling, energy transfer, quantitation, and photobleaching experiments. Results from this work are clarifying the steps involved in the formation, translocation, and fusion of transport intermediates; the organization and biogenesis of organelles; and the mechanisms of protein retention, sorting, and recycling in the secretory pathway. In so doing, they are broadening our thinking about the temporal and spatial relationships among secretory organelles and the membrane trafficking pathways that operate between them.

PMID:
11031247
PMCID:
PMC4781643
DOI:
10.1146/annurev.cellbio.16.1.557
[Indexed for MEDLINE]
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