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Curr Opin Biotechnol. 2000 Oct;11(5):440-4.

In vivo expression technology strategies: valuable tools for biotechnology.

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Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB, Oxford, UK.


Whole genome sequences have shown that bacteria possess a significant number of genes that have no known function. It is probable that many of these are required for survival in environments other than the agar plate. In vivo selection strategies provide a means of obtaining genes active in complex natural environments. Direct access to these genes is essential for understanding ecological performance and provides novel opportunities for biotechnology.

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